学歴

1993年3月　日本大学農獣医学部農芸化学科卒業
1995年3月　日本大学大学院農学研究科博士前期課程農芸化学専攻修了
1998年3月　日本大学大学院農学研究科博士後期課程農芸化学専攻修了

略歴

1998年4月　　　　　　　日本大学医学部助手
2000年4月～2002年8月　 シンシナティ大学メディカルセンター客員研究員
2006年6月　　　　　　　日本大学医学部専任講師
2024年9月　　　　　　　盛岡大学栄養科学部教授

学位

博士（農学）

専⾨分野

生化学・毒性学

研究テーマ

食品成分によるがん予防

担当科目

生化学

主な研究業績

著書

• １．非栄養素の分子栄養学　第1編　解毒代謝・炎症の分子栄養学　第2章　ニンニク成分の遺伝子発現を介した生理作用　2017/5/10　建帛社　共著
• ２．[学会・シンポジウム見聞録]　ゲノム医学の次なるステップ“トランスレーショナルリサーチ”（第４回RGCMフロンティアシンポジウム）実験医学1822-1823、 Vol.24 No.12、2006羊土社

論文

• 1. Adachi K, Ishizawa M, Uno S, Kubota H, Henmi T, Koshinaga T, Makishima M, Sakurai K. Oral benzo[a]pyrene administration attenuates dextran sulfate sodium-induced colitis in mice. Chemico-Biological Interactions 353:109802, 2022
• 2. Endo-Umeda K, Nakashima H, Uno S, Toyoshima S, Umeda N, Komine-Aizawa S, Seki S, Makishima M. Liver X receptors regulate natural killer T cell population and antitumor activity in the liver of mice. Scientific Reports 11(1):22595, 2021
• 3. Maekawa T, Kato S, Kawamura T, Takada K, Sone T, Ogata H, Saito K, Izumi T, Nagao S, Takano K, Okada Y, Tachi N, Teramoto M, Horiuchi T, Hikota-Saga R, Endo-Umeda K, Uno S, Osawa Y, Kobayashi A, Kobayashi S, Sato K, Hashimoto M, Suzu S, Usuki K, Morishita S, Araki M, Makishima M, Komatsu N, Kimura F. I ncreased SLAMF7high monocytes in myelofibrosis patients harboring JAK2V617F provide a therapeutic target of elotuzumab. Blood. 134(10):814-825, 2019
• 4. Uno S, Sakai M, Fujinari Y, Hosono T, Seki T, Makishima M. Diallyl trisulfide enhances benzo[a]pyrene-induced CYP1A1 expression and metabolic activation in hepatic HepG2 cells. Anticancer Research. 39(5):2369-2375, 2019
• 5. Uno S, Shimizu T, Tanaka T, Ashiba H, Fujimaki M, Tanaka M, Awazu K, Makishima M. Application of a Waveguide-Mode Sensor to Blood Testing for Hepatitis B Virus, Hepatitis C Virus, Human Immunodeficiency Virus and Treponema pallidum Infection. Sensors (Basel, Switzerland). 19(7): 1729, 2019
• 6. Nakagawa M, Uno S, Iriyama N, Matsunawa M, Makishima M, Takeuchi J, Tsuboi I, Hatta Y, Takei M. Combined treatment with benzo[a]pyrene and 1α,25-dihydroxyvitamin D3 induces expression of plasminogen activator inhibitor 1 in monocyte/macrophage-derived cells. Toxicology and applied pharmacology 345:48-56, 2018
• 7. Uno S, Tanaka T, Ashiba H, Fujimaki M, Tanaka M, Hatta Y, Takei M, Awazu K, Makishima M. Sensitive typing of reverse ABO blood groups with a waveguide-mode sensor. Journal of bioscience and bioengineering 126(1):131-137 2018
• 8. Uno S, Nebert DW, Makishima M. Cytochrome P450 1A1 (CYP1A1) protects against nonalcoholic fatty liver disease caused by Western diet containing benzo[a]pyrene in mice. Food and chemical toxicology 113:73-82, 2018
• 9. Shimizu T, Tanaka T, Uno S, Ashiba H, Fujimaki M, Tanaka M, Awazu K, Makishima M. Detection of antibodies against hepatitis B virus surface antigen and hepatitis C virus core antigen in plasma with a waveguide-mode sensor. Journal of Bioscience and Bioengineering 123(6):760-764, 2017
• 10. Watarai Y, Ishizawa M, Ikura T, Zacconi FC, Uno S, Ito N, Mouriño A, Tokiwa H, Makishima M, Yamada S. Synthesis, Biological Activities, and X-ray Crystal Structural Analysis of 25-Hydroxy-25(or 26)-adamantyl-17-[20(22),23-diynyl]-21-norvitamin D Compounds. Journal of medicinal chemistry 58(24):9510-21, 2015
• 11. Cázares-Ordoñez V, González-Duarte RJ, Díaz L, Ishizawa M, Uno S, Ortíz V, Ordoñez-Sánchez ML, Makishima M, Larrea F, Avila E. A cis-acting element in the promoter of human ether à go-go 1 potassium channel gene mediates repression by calcitriol in human cervical cancer cells. Biochemistry and Cell Biology 93(1):94-101, 2015
• 12. Uno S, Sakurai K, Nebert DW, Makishima M. Protective role of cytochrome P450 1A1 (CYP1A1) against benzo[a]pyrene-induced toxicity in mouse aorta. Toxicology 316: 34-42, 2014
• 13. Nebert DW, Shi Z, Gálvez-Peralta M, Uno S, Dragin N. Oral Benzo[a]pyrene: Understanding Pharmacokinetics, Detoxication, and Consequences--Cyp1 Knockout Mouse Lines as a Paradigm. Molecular Pharmacology 84(3):304-313, 2013
• 14. Matsunawa M, Akagi D, Uno S, Endo-Umeda K, Yamada S, Ikeda K, Makishima M. Vitamin D Receptor Activation Enhances Benzo[a]pyrene Metabolism via CYP1A1 Expression in Macrophages. Drug metabolism and disposition: the biological fate of chemicals 40(11): 2059-2066, 2012
• 15. Kakuta H, Yakushiji N, Shinozaki R, Ohsawa F, Yamada S, Ohta Y, Kawata K, Nakayama M, Hagaya M, Fujiwara C, Makishima M, Uno S, Tai A, Maehara A, Nakayama M, Oohashi T, Yasui H, Yoshikawa Y. RXR Partial Agonist CBt-PMN Exerts Therapeutic Effects on Type 2 Diabetes without the Side Effects of RXR Full Agonists. ACS medicinal chemistry letters 3(5):427-32, 2012
• 16. Endo-Umeda K, Uno S, Fujimori K, Naito Y, Saito K, Yamagishi K, Jeong Y, Miyachi H, Tokiwa H, Yamada S, Makishima M. Differential expression and function of alternative splicing variants of human liver X receptor α. Molecular Pharmacology 81(6):800-10, 2012
• 17. Ozeki J, Uno S, Ogura M, Choi M, Maeda T, Sakurai K, Matsuo S, Amano S, Nebert DW, Makishima M. Aryl hydrocarbon receptor ligand 2,3,7,8-tetrachlorodibenzo-p-dioxin enhances liver damage in bile duct-ligated mice. Toxicology 280(1-2):10-7, 2011
• 18. Shi Z, Dragin N, Miller ML, Stringer KF, Johansson E, Chen J, Uno S, Gonzalez FJ, Rubio CA, Nebert DW. Oral benzo[a]pyrene-induced cancer: two distinct types in different target organs depend on the mouse Cyp1 genotype. International Journal of Cancer 127(10):2334-50, 2010
• 19. Uno S, Endo K, Ishida Y, Tateno C, Makishima M, Yoshizato K, Nebert DW. CYP1A1 and CYP1A2 expression: comparing ‘humanized’ mouse lines and wild-type mice; comparing human and mouse hepatoma-derived cell lines. Toxicology and Applied Pharmacology 237(1): 119-126, 2009
• 20. Matsunawa M, Amno Y, Endo K, Uno S, Sakaki T, Yamada S, Makishima M. The aryl hydrocarbon receptor activator benzo[a]pyrene enhances vitamin D3 catabolism in macrophages. Toxicological Sciences 109(1): 50-58, 2009
• 21. Dong H, Dalton TP, Miller ML, Chen Y, Uno S, Shi Z, Shertzer HG, Bansal S, Avadhani NG, Nebert DW. Knock-in mouse lines expressing either mitochondrial or microsomal CYP1A1: differing responses to dietary benzo[a]pyrene as proof of principle. Molecular Pharmacology 75(3): 555-567, 2009
• 22. Ogura M, Nishida S, Ishizawa M, Sakurai K, Shimizu M, Matsuo S, Amano S, Uno S, Makishima M. Vitamin D3 Modulates the Expression of Bile Acid Regulatory Genes and Represses Inflammation in Bile Duct-Ligated Mice. Journal of Pharmacology and Experimental Therapeutics 328(2): 564-570, 2009
• 23. Uno S, Endo K, Jeong Y, Kawana K, Miyachi H, Hashimoto Y, Makishima M. Suppression of β-catenin signaling by liver X receptor ligands. Biochemical pharmacology 77(2): 186-195, 2009
• 24. Nakagawa A, Uno S, Makishima M, Miyachi H, Hashimoto Y. Progesterone receptor antagonists with a 3-phenylquinazoline-2,4-dione/2-phenylisoquinoline-1,3-dione skeleton. Bioorganic & Medicinal Chemistry 16(14):7046-7054, 2008
• 25. Endo K, Uno S, Seki T, Ariga T, Kusumi Y, Mitsumata M, Yamada S, Makishima M.　Inhibition of aryl hydrocarbon receptor transactivation and DNA adduct formation by CYP1 isoform-selective metabolic deactivation of benzo[a]pyrene. Toxicology and applied pharmacology 230(2):135-143, 2008
• 26. Ishizawa M, Matsunawa M, Adachi R, Uno S, Ikeda K, Masuno H, Shimizu M, Iwasaki KI, Yamada S, Makishima M. Lithocholic acid derivatives act as selective vitamin D receptor modulators without inducing hypercalcemia. Journal of lipid research 49(4):763-772, 2008
• 27. Uno S, Dragin N, Dalton TP, Miller ML, Gonzalez FJ, Nebert DW. Basal and inducible CYP1 mRNA quantitation and protein localization throughout the mouse gastrointestinal tract. Free Radical Biology & Medicine 44(4):570-583, 2008
• 28. Dragin N, Uno S, Wang B, Dalton TP, Nebert DW. Generation of 'humanized'　hCYP1A1_1A2_Cyp1a1/1a2(-/-) mouse line. Biochemical and Biophysical Research Communications 359(3): 635-642, 2007
• 29. Inaba Y, Yamamoto K, Yoshimoto N, Matsunawa M, Uno S, Yamada S, Makishima M. Vitamin D3 derivatives with adamantane or lactone ring side chains are cell type-selective vitamin D receptor modulators. Molecular Pharmacology 71(5):1298-1311, 2007
• 30. Koike T, Uno S, Ishizawa M, Takahashi H, Ikeda K, Yokoto S, Makishima M. The heat shock protein inhibitor KNK437 induces neurite outgrowth in PC12 cells. Neuroscience Letters 410(3): 212-217, 2006
• 31. Uno S, Dalton TP, Dragin N, Curran CP, Derkenne S, Miller ML, Shertzer HG, Gonzalez FJ, Nebert DW. Oral benzo[a]pyrene in Cyp1 knockout mouse lines: CYP1A1 important in detoxication, CYP1B1 metabolism required for immune damage independent of total-body burden and clearance rate. Molecular Pharmacology 69(4): 1103-1114, 2006
• 32. Uno S, Dalton TP, Derkenne S, Curran CP, Miller ML, Shertzer HG, Nebert DW. Oral exposure to benzo[a]pyrene in the mouse: detoxication by inducible CYP1A1 more important than metabolic activation. Molecular Pharmacology 65(5):1225-1237, 2004
• 33. Uno S, Dalton TP, Sinclair PR, Gorman N, Wang B, Smith AG, Miller ML, Shertzer HG, Nebert DW. Cyp1a1(-/-) male mice: protection against high-dose TCDD-induced lethality and wasting syndrome, and resistance to intra-hepatocyte lipid accumulation and uroporphyria. Toxicology and Applied Pharmacology 196(3): 410-421, 2004
• 34. Uno S, Wang B, Shertzer HG, Nebert DW, Dalton TP. Balancer-Cre transgenic mouse germ cells direct the incomplete resolution of a tri-loxP-targeted Cyp1a1 allele, producing a conditional knockout allele. Biochemical and Biophysical Research Communications 312(2): 494-499, 2003
• 35. Watanabe Y, Takahashi H, Uno S, Takano S, Nakagawa S. Expression of type 1 plasminogen activator inhibitor and nitric oxide generation in ischemic preconditioning of rat liver. Hepatology Research 25(3): 343-53, 2003
• 36. Senft AP, Dalton TP, Nebert DW, Genter MB, Puga A, Hutchinson RJ, Kerzee JK, Uno S, Shertzer HG: Mitochondrial reactive oxygen production is dependent on the aromatic hydrocarbon receptor. Free Radical Biology & Medicine 33(9):1268-78, 2002
• 37. Uno S, Dalton TP, Shertzer HG, Genter MB, Warshawsky D, Talaska G, Nebert DW: Benzo[a]pyrene-induced toxicity: paradoxical protection in Cyp1a1(-/-) knockout mice having increased hepatic BaP-DNA adduct levels. Biochemical and Biophysical Research Communications 289(5): 1049-1056, 2001
• 38. Noguchi T, Matsuyama S, Akao M, Hagiwara H, Uno S, Seki T, Ariga T: Induction of hepatic tissue-type plasminogen activator and type 1 plasminogen activator-inhibitor gene expressions and appearance of their translation products in the bile following acute liver injury in rats. Thrombosis Research 104 (4) 283-291, 2001
• 39. Takahashi H, Uno S, Watanabe Y, Arakawa K, Nakagawa S: Expression of nerve growth factor-induced type 1 plasminogen activator inhibitor (PAI-1) mRNA is inhibited by genistein and wortmannin. Neuroreport 11(5): 1111-1115, 2000
• 40. Uno S, Nakamura M, Ohomagari Y, Matsuyama S, Seki T, Ariga T: Regulation of tissue-type plasminogen activator (tPA) and type-1 plasminogen activator inhibitor (PAI-1) gene expression in rat hepatocytes in primary cultured. Journal of biochemistry 123(5): 806-812, 1998
• 41. Uno S, Nakamura M, Seki T, Ariga T: Induction of tissue-type plasminogen activator (tPA) and type-1 plasminogen activator inhibitor (PAI-1) as early growth responses in rat hepatocytes in primary culture. Biochemical and Biophysical Research Communications 239(1): 123-128, 1997
• 42. eki T, Imai H, Uno S, Ariga T, Gelehrter TD: Production of tissue-type plasminogen activator (tPA) and type-1 plasminogen activator inhibitor (PAI-1) in mildly cirrhotic rat liver. Thrombosis and Haemostasis 75(5):801-807, 1996

受賞

• 2013年4月20日　James R. Gillette Best Paper Award　(Experimental Biology 2013, Aspet)